Microfluidic Plates
There are three different types of plates; Yeast, Mammalian and Bacterial. All three versions have four independent flow units (with identical flow properties) and allow simultaneous imaging of four sets of cell/medium combinations. Perfusion culture allows cells to be kept alive for at least 3 days, which is also helped by the plates only needing tiny amounts of liquids. There is an option of temperature and gas control also available for each plate.
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Yeast Microfluidic Plates
The Yeast Microfluidic plates are optimised for time lapsed imaging of yeast cells with solution exchange. The yeast plates have a unique cell trapping region that holds the yeast cells in a uniform focal plane for time-lapse cell microscopy during perfusion flow. Four upstream solution reservoirs allow rapid switching of the exposure solution during time-lapsed imaging to monitor real time cell responses.
The Y-series plates offer variable trapping heights, as well as special plates for Diploid yeast. Tracking daughter cells is easy due to cells dividing in the same (in focus) plane, which in turn is trapped in between 2 optical glass cover-slips. Another benefit of the trapped cells is that they don't move in and out of focus meaning that they are ideal for long time-lapse movies.
Features and Benefits
- Elastic trap sites holds cells in single foal plane for 16+ hours
- 4 independant chambers under a single imaging window
- Laminar flow switching between 6 inlet solutions
- 170 um thick glass bottom for high quality imaging.
Yeast plate variants
Y04C - Y04C-02 Yeast Perfusion Plate (3.5-5 um)
Y04D - Y04D-02 Yeast Perfusion Plate (5-7 um)
- Fig.1 overhead labled diagram of plate
- Fig.2 reconstruction of live yeast cells
Mammalian Microfluidic Plates
The M04 plates enable continuous perfusion culture for live cell imaging. Laminar flow provides rapid and uniform solution exchange for maximum cell health. The glass coverslide bottom surface ensures the highest resolution optical images. | |||||||
Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-72 hours on the microscope stage, enabling single cell tracking during exposure, washout, and switching. Cells are easily loaded via pipet, and can be cultured for many days prior to experiment using a gravity driven flow in any incubator.
Mammalian Plate Variants M04S - M04S-03 Perfusion Switching Plate M04L - M04L-03 Open-top Switching Plate (formerly M04O) M04G - M04G-02 Gradient Plate (2D and 3D)
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Bacterial Microfluidic Plates
The B04A plates utilize a microfabricated silicone ceiling with a height similar to bacteria cells to restrict their growth in a single focal plane and maintain x,y position over time. Each chamber contains 6 tapered trap heights to maximize loading efficiency of various cell sizes. The B04A plate has heights of 0.7, 0.9, 1.1, 2.0, 3.0 and 4.0 um. (See Product Sheet for more detail.) | ||
Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-16 hours on the microscope stage, enabling single cell tracking for over 10 doublings. The six inlets to each chamber allow solution switching experiments during time lapsed imaging. The highly laminar flow results in a rapid and complete solution change in under a minute.
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