Expertise in scientific imaging

DeltaVision OMX SR

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DeltaVision OMX SR Super-resolution Microscope

 
 
 

 

The DeltaVision OMX SR is a compact imaging system specifically optimized to provide a stable platform for structured illumination microscopy (SIM) super resolution technology.

 

  • See beyond the norm – 3D SIM enables resolution improvements in x, y, and z directions.
  • Super-resolve live cells – 2D SIM and 2D SIM-TIRF imaging modes allow for dynamic super-resolution live-cell imaging.
  • Molecular imaging – localization microscopy option uses exclusive 2D multi-emitter fitting algorithms to reconstruct a super-resolved image.
  • More data from your sample – multiplex data acquisition with simultaneous multi-camera imaging.
  • Preserve cell viability – ultra-low light imaging combined with advanced deconvolution algorithm.
  • Capture dynamic processes – rapid image acquisition and stage automation.

Overview

Designed for biologists wishing to advance their research beyond what is possible with confocal microscopes, DeltaVision OMX SR improves spatial resolution by a factor of 2 in x, y, and z directions, enabling previously unseen detail to be obtained even in live samples.

DeltaVision OMX SR is fully enclosed, does not require a darkroom, and can be installed ready-to-run in a standard lab within days. And unlike many super-resolution systems, there is minimal change required for sample preparation or preferred buffers and fluorophores.

Same methods, super-resolution results

DeltaVision OMX SR is the latest addition to the high-performing DeltaVision portfolio and builds on the reputation of DeltaVision Elite and DeltaVision OMX for delivering outstanding images particularly for small, dim and live samples. In addition to 2D and 3D SIM super-resolution imaging, the DeltaVision OMX SR is also capable of widefield, time-lapse imaging, deconvolution, and TIRF illumination using the same techniques and fluorophores utilized on confocal imagers. For added flexibility, a ring TIRF option provides even illumination and artifact-free TIRF imaging enabling the system to be used for localization microscopy and photokinetic applications.

For biologists seeking a more expandable platform, the DeltaVision OMX also supports multiple laser configurations and additional camera options.

PRODUCT SPECIFICATIONS

DeltaVision OMX SR – Product Specifications

 
ParameterDeltaVision OMX SR
CameraManufacturer PCOChip type Front illuminated sCMOSChip size: 2560 × 2160 pixels* (Useable area limited to 1024 x 1024 for SIM imaging)Pixel size: 6.5 µmReadout speeds: 95 MHz, 286 MhzReadout modes: Rolling shutter, global shutterCamera interface: Camera-linkBit depth: 15 bitQuantum efficiency: ~ 60%Dynamic range: 1:15 000Read noise: 1.5 e at 33 fps, 2 e at 100 fpsOn-chip binning: Not supported 
FocusLaser based hardware autofocusCoarse/Long travel focus- Nanomover stepper motorFine/High speed focus – Piezo actuator 
MagnificationSuper-resolution mode requires 60x to support camera configuration.Optional magnifications for widefield and TIRF imaging modes. 
Plate Positioning (XY Stage)Stage range (xy): 24 × 48 mmy flatness: < 1 µm/mm*x flatness: < 1 µm/mm*xyz Nanomover repeatability: 10 µm/25 mm travelz piezo step resolution: 5 nmxyz Nanomover step resolution: 100 nmSample holder types Standard: 75 × 25 mm slides, 35 mm dishes, Multichamber coverslips 
Filter SelectionStandard filters for DAPICFPFITC/AF488YFPAF568/Texas RedCy5/AF647 
Plate CompatabilityNot Applicable 
ComputerOMX Master Workstation CPU Intel Core™ i7 processorClock speed 3.4 GHzMemory 16 GB DDR3 1333 MHz ECCPower supply 500 WDrives 1 TB SATA boot driveRAID 3 × 1 TB SATA in RAID5 configurationVideo card NVidia™ NVS300 VRAM 512 MBVideo bus PCIEMonitor panel size 24 in (60.96 mm) viewable areaResolution 1920 × 1200Operating system CentOS 6 or laterSpecifications subject to change without notice 
Height1575 mm 
Width864 mm
Depth991 mm 
WeightApproximately 480 kg
ObjectivesStandard Lenses60x 1.42 NA Oil Immersion PSF for SIM Imaging60x 1.49 NA TIRF for TIRF imagingOptional objectives20x/ 0.75 Super-Plan APO40x/ 1.35 Oil Plan APO60x/ 1.3 Silicone Plan APO100x/ 1.4 Oil Super-Plan APO100x/ 1.49 TIRF APO 
Optical System2 Illumination input paths for 3D-SIM/2D-SIM/2D-SIM-TIRF/Widefield and, Ring-TIRF/PK/Widefield modesUp to 4 camera detection channels 
Slide CompatibilityStandard microscope slides35 mm glass bottom dishesMultiwell chambered coverslips 
SoftwareOMX Master Control Software – AcquireSRsoftWoRx reconstruction and analysis software 
Light SourceMulticolor laser illumination 
Imaging ModesFluorescence imaging with deconvolutionDIC imaging3D-SIM super-resolution2D-SIM super-resolution2D-SIM TIRF super-resolutionLocalization microscopy super-resolution (e.g. PALM, STORM) 
Image Acquisition FeaturesMultiple camera capabilitySuper-resolution imagingWidefield imagingDIC imaging3D sectioningTimelapse imagingCell trackingMultipoint imaging 
Optional HardwareDeltaVision Localization Microscopy System (29-0876-63 )Environmental Control Module and Live-Cell Kit (29-1330-64)Universal Long Working Distance (LWD) Condenser Assembly with DIC Prism Holder Ring (29-1330-63)Offline SI Workstation (53-853053-000)