Expertise in scientific imaging

Mightex Oasis Implant

Understand the Link Between Neural Circuits and Behaviour

The OASIS Implant is a ground-breaking platform for simultaneous cellular-resolution optogenetics and calcium imaging in freely-behaving animals to probe complex neuronal networks
  • Simultaneous Cellular-Resolution Calcium Imaging and Optogenetics
  • Multi-Region Investigation
  • Reconfigurable Platform
  • High-Quality Imaging with Scientific Cameras

The Evolution in Freely-Behaving Imaging and Optogenetics Technology

To offer neuroscientists the ability to stimulate and visualize individual neurons in freely-behaving animals, Mightex’s engineers went back to the drawing board and pioneered a new path in the in vivo calcium imaging field. Instead of miniaturizing and placing the microscope on the animal – thereby limiting the microscope’s features and the animal’s behaviour – we kept the microscope off the animal’s head by using an imaging fiber.

 

By keeping the microscope away from the animal’s head, the OASIS Implant accomplishes 2 main goals:

 

OASIS Implant Key Features

Investigate Multiple Brain Regions Simultaneously

Neurons project to different parts of the brain, and neuroscientists are interested in understanding how these connections affect behaviourThe OASIS Implant uses a bifurcated imaging fiber that connects to multiple GRIN lenses, allowing researchers to perform imaging and optogenetics in multiple brain regions simultaneously.

Manipulate Functional Neuronal Activity with Cellular-Resolution Optogenetics

To dissect neural circuits at the level of individual neurons, neuroscientists need a tool to perform cellular-resolution optogenetics in freely-behaving animals. The OASIS Implant uses Mightex’s Polygon DMD Illuminator to illuminate individual neurons in vivo.

implant_opto_2

GCaMP in vivo calcium imaging in a mouse using the OASIS Implant (Courtesy of Alexxai Kravitz, Washington University in St. Louis).

Fiber Photometry and Cellular-Resolution Calcium Imaging with One System

The OASIS Implant is capable of both population-level calcium imaging using fiber photometry and cellular-resolution calcium imaging. Perform exploratory research using fiber photometry, and delve in deeper to understand the patterns of individual neuron firing.

Micro- to Meso-Scale Imaging and Optogenetics

The OASIS Implant enables neuroscientist to perform imaging and optogenetics with cellular-resolution in a deep-region of the brain, or image a large cortical region and stimulate specific populations of neurons (0.5mm to 3.2mm FoV).

Link Neural Activity and Behaviour

Neuroscientists are interested in linking brain activity and behaviour. Using the OASIS Implant, imaging and optogenetics data can be synchronized with external behaviour cameras and behaviour equipment using the included IO control box to link neural activity and behavioural events.

Compatible with Scientific-Grade Cameras

 
 
 

Rotation Compensation Option for Freely-Behaving Experiments

Reconfigurable System that can be Tailored for Different Experiments

 
 

Light-Weight Headmount to Reduce Strain on Animal Behaviour

Intuitive Signal Processing for Data Analysis

 

1. ACQUIRE IN VIVO CALCIUM RECORDINGS

The software provides a complete set of tools to allow researchers to visualize and capture calcium imaging recordings. The software includes features to synchronize the calcium recordings with the rest of the behavioral equipment and cameras used in the experiment.

OASIS Implant Headmounts

 

Standard Headmount

 

Compact Headmount

OASIS Implant Imaging Probes

 

Deep-Brain Imaging Probes

 

Cortical Large Field of View Imaging Probe

This flexible imaging probe is designed for large field of view applications (up to 3.2mm) across the cortex by using a fiber with an increased field of view through a cortical window.

 
PART #RESOLUTIONFIELD-OF-VIEWLENGTH
FBR-0650-30K-XXX-10X3 um0.65 mm1-3 meters
FBR-0650-30K-XXX-34X10.2 um2.2 mm1-3 meters
FBR-0650-30K-XXX-50X15 um3.2 mm1-3 meters
 

Multi-Region Imaging Probe

This flexible imaging probe is designed for multi-region imaging and optogenetics applications by using a split-fiber coupled to multiple implanted GRIN lenses.

 
PART #RESOLUTIONFIELD-OF-VIEWLENGTH
FBR-F2-0350-10K-XXX-XX3 um350um1-3 meters
 

Soft Imaging Probe

This soft imaging probe is designed for applications requiring increased mobility, such as songbirds, by using a flexible fiber coupled to an implanted GRIN lens.

 
PART #RESOLUTIONFIELD-OF-VIEWLENGTH
FBRS-1000-18K-075-0T7.6 um1000 um0.75 meters

OASIS Publications