Expertise in scientific imaging

Microfluidic Plates for Yeast Cells

Microfluidic Plates for Yeast Cells

  • The Y-Series microfluidic plates keep yeast cells in a single focal plane, allowing you to follow and induce cell events during high magnification analysis over many generations.Features and Benefits:
    • Elastic trap sites holds cells in single focal plane for 16+ hours
    • Laminar flow switching between 6 inlet solutions

    The Y04 plates utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane and maintaining x,y position over time. Each chamber contains 3 tapered trap heights to maximize loading efficiency of various cell sizes. The Y04C plate has heights of 3.5, 4.5 and 5.0 µm, and the Y04E plate has heights of 5, 6, 7 µm. (See Product Sheet for more detail.)

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-16 hours on the microscope stage, enabling single cell tracking for over 10 doublings. The six inlets to each chamber allow solution switching experiments during time lapsed analysis. The highly laminar flow results in a rapid and complete solution change in under a minute.

Cell division of S. cerevisiae over 15 hours with continuous flow in the Y04 microfluidic plate. Note the uniformity of focal plane during the entire period. Views taken with a 40x phase objective.

CellASIC ONIX Y04C plate for haploid yeast cells (4 chamber, 3.5-5 micron)

 
 
The Y04C plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.
 
 
Description
Catalogue NumberY04C-02-5PK
Trade Name
  • CellASIC ONIX
DescriptionCellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron)

Product Information
Device Configuration4 chamber

Applications
ApplicationThe Y04C plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

Dimensions
Dimensions85.48 mm Wide x 127.76 mm Long x 14.35 mm Height
Cell Size Range3.5–5.0 µm

Materials Information
Device Material
  • PDMS
  • PMMA
  • Glass
  • Polycarbonate

Packaging Information
Material Size5
 

User Guide

CellASIC ONIX Y04E plate diploid yeast cells (4 chamber, 5-7 micron)

The Y04E plates for Diploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

Description
Catalogue NumberY04E-02-5PK
Trade Name
  • CellASIC ONIX
DescriptionCellASIC ONIX plate diploid yeast cells (4 chamber, 5-7 micron)

Product Information
Device Configuration4 chamber

Applications
ApplicationThe Y04E plates for Diploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

Dimensions
Dimensions85.48 mm Wide x 127.76 mm Long x 14.35 mm Height
Cell Size Range5.0–7.0 µm

Materials Information
Device Material
  • PDMS
  • PMMA
  • Glass
  • Polycarbonate

Packaging Information
Material Size5
 

User Guide

CellASIC ONIX Y04D Microfluidic Plates User Guide

 

CellASIC ONIX Y04T Pad Trap Plate (4-chamber, trap heights 4 micron), 5pk

The Y04T pad trap plates for 4 µm cells utilize microfabricated PDMS pillars that extend from the ceiling to selectively trap cells and restrict their growth in a single focal plane & maintain x,y positioning over time.

Description
Catalogue NumberY04T-04-5PK
Trade Name
  • CellASIC ONIX
DescriptionCellASIC ONIX Pad Trap Plate (4-chamber, trap heights 4 micron), 5pk
Background InformationBarrier structures retain cells while enabling perfusion to wash away smaller cells.

Product Information
Device Configuration4 chamber

Applications
ApplicationThe Y04T pad trap plates for 4 µm cells utilize microfabricated PDMS pillars that extend from the ceiling to selectively trap cells and restrict their growth in a single focal plane & maintain x,y positioning over time.

Dimensions
Dimensions6.0 mm length x 3.0 mm width with trap heights of 4.0 µm
Cell Size Range3.5–5.0 µm

Materials Information
Device Material
  • PDMS
  • PMMA
  • Glass
  • Polycarbonate

Packaging Information
Material Size5
 

User Guide