Image Solutions Launch A New Quantifiable Laser Module For FRET, FRAP and PA-GFP Applications
Preston based Image Solutions Ltd, are launching a new quantifiable laser module option to work with the DeltaVision Spectris system for image restoration microscopy.
The DeltaVision Spectris is particularly effective at imaging small, low contrast, weakly fluorescent specimens and then making quantitative comparisons of cellular components via time-lapse studies of cell motility, intracellular mechanics and molecular movement. The DeltaVision Spectris also excels in collecting signals from specimens with minimal photobleaching, which is often a critical concern for fixed-cell samples.
The DeltaVision Spectris is highly flexible and with the new laser module it can accommodate many different experiments including FRET (Fluorescence Resonance Energy Transfer), FRAP (Fluorescence Recovery After Photobleaching) and PA-GFP (Photo Activated Green Flourescent Protein).
The Image is captured by DeltaVision Spectris. The image shows dynamic localisation of GFP-SpoIIQ (green) during engulfment of spore formation in Bacillus Subtilis. The image is based on micrographs provided by Aileen Rubio, a postdoctoral fellow in the Laboratory of Kit Pogliano, Division of Biological Sciences at The University of California, San Diego.
The proprietary Nanomotion stage is an essential component of Deltavision’s 3D imaging technology. No other stage matches its accuracy and repeatablility over its full range of motion. Without highly accurate and repeatable stage motion, the mathematical requirements for 3D deconvolution are undermined and the quality of the deconvolved image is degraded. Time after time DeltaVision has been shown to acquire superior 3D images.
10/11/2004