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Microscope Accessories

We supply microscopes and imaging accessories for all applications, including the design and fabrication of custom parts where necessary. Below are just a few of the items that we can supply, so contact us if you don't see the part that you are looking for.

  • Light sources e.g. Cold, Xenon, Mercury, Xe/Hg hybrids and monochromators
  • Specialist filters and sets
  • Filter wheels and filter changers
  • Heated & cooled stages
  • Environmental chambers with CO2 enrichment/heaters
  • Custom workbenches
  • Anti vibration solutions & air tables
  • Perfusion pumps
  • Flow chambers
  • Specialist camera couplers
  • Micro-injection and micro-manipulation
  • Immersion oils
  • Replacement EXFO X-Cite 120 bulbs for illumination systems 
A sealed bottle of immersion oil - 1 fl. ounce

Immersion and Laser Oils

Immersion oils allow the camera to view a higher resolution by increasing the numerical aperture to around 1.42, instead of just 1.0 with air alone. The oil works by coupling the end of the objective and coverslip of the sample, which gives both higher resolution as well as capturing more light.

 

We can offer a range of oils with different refractive indexes which we can match to the temperature of the sample you are using. The oils we stock range from 1.510 up to 1.534.

A range of camera couplers

Camera Couplers

Upgrading your camera coupler is a simple way to boost your current system's performance. The Diagnostic Instruments camera couplers that we supply can connect almost every camera to any scope. The couplers come in two parts, one which attaches to the camera, another to the microscope. There are also a range of chips we supply; 1/3 inch, 1/2 inch, 2/3 inch, 1 inch and 1 1/3 inch. Matching the chip size to the coupler mag is recomended.

 

 

CO2 Controller

The Brooks Mode 5850E Mass Flow Controller accurately measures and controls gas flow. The heart of the system is the removable flow sensor which produces an electrical output signal linear with flow rate used for indicating, recording, and/or control purposes. It eliminates the need for continuous monitoring and readjustment of gas pressures to provide a stable gas flow.

 

 

Dual wheel filter set magazine, arranged to show both wheels.

Filters and Filter Sets

The main feature of a fluorescence microscope is to provide a medium for excitation of the subject you want to view, by using a secondary filter. The combination of the two filters enables an image to be seen on a dark background, with maximum sensitivity. We stock a range of filters and filter sets, made by both Semrock and Chroma. We are also able to supply a dual wheel filter set, meaning you can switch between filter sets almost instantaneously.

A mercury bulb in its packaging
Xenon bulb in housing
Mercury bulb in its packaging

Light Sources

There are a range of options when it comes to lighting for your microscopes. Listed below are the options that we can offer:

Xenon bulbs - a high power lamp in a ceramic packet. These bulbs work straight out of the box, and don't require adjustment. They are guaranteed to last 2000 hours.

Mercury bulbs - are a more traditional lighting system. They require adjustment when fitting, and last for about 300 hours.

Cold light sources - can provide a variable intensity white light for use with a stereo microscope. Cold light sources filter out UV rays so that any organism being viewed will not be affected.

Hybrids - these are metal halide lamps. They are unpressurised and last aroud 2000 hours.

Monochramators - these transmit a mechanically selectable band of wavelengths. They are typically controlled by an analogue voltage which equates to the wavelength that is needed. The main advantage of a monochromator is that they can change between wavelenghts extremely quickly. However, they are not as bright as normal filtered lights

Environmental Chambers

Environmental Chamber at Sheffield University

Envronmental chambers are vital if you want to carry out an experiment over an extended period. The chamber keeps the environment at a specified temperature and uses a PID controller to ensure the temperature stays constant. Environmental chambers are also less sensitive to external temperature changes than a stage or objective heater, as there is a cushion of air around the microscope which helps absorb the change and stops it reaching the sample. The body of the microscope also acts as a thrmal mass, helping to minimise temperature fluctuations.

The chamber is ideal for cells that need humidifie CO2 to stay alive, as the chamber has a hydrating column inside. The column works by bubbling the CO2 through the water, before it goes to a small stage top chamber which becomes flooded with CO2. The CO2 can be supplied in pre-mixed bottles (5%), or by a gas mixer that takes CO2 from the bottle and mixes it with air from the room to make a (5%) mix.

Environmental chambers are the best solution to live cell imaging.

Heated and Cooled stages

Heated stages are an alternative for keeping your sample at a certain temperature. They contain a heating element and have a thermocouple to control the temperature. There are two types of heated stage, these are 37 deg. and variable temperature. These are not suitable for long time lapse experiments.

Cooled stages can be used for liquid crystal experiments, and use a powerful heatsink/fan combination to cool the element.

Objective heaters keep the objective at the same temperature as the sample so that it does not draw heat away. There is an objective heater controller that allows you to set and adjust the temperature.

Imsol Anti-Vibration Table

Image Solutions have designed an aftermarket anti-vibration table for the AppliedPrecision personalDV (pDV). Although the pDV does come with an anti-vibration plinth, some customers require the added protection offered by the table. The table is set on special feet, which can block out vibrations as low as 20Hz.

Micro Injectors/Manipulators

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Image Solutions are pleased to announce that we now offer micro-manipulator and injector support for the entire range of DeltaVision restoration systems.

 

In association with our partners at Eppendorf, we can offer a twin mount solution for integration into all existing environmental chambers (Solent and WeatherStation) for your requirements. The mounts are designed for full stage operation, full home-relaxation position of the probe/injector and is highly stable and vibration free.

 

Integration of the micro-injection can be made with Applied Precisions SoftWorx. We offer consultancy and training to allow the  full deployment of these devices with time-lapse studies in mind.

 

Please contact Image Solutions to discuss your requirements. Tailor-made solutions are also considered during the integration process (including Burleigh)-and physiology-based applications involving the DeltaVison will be undertaken.

TIRF System (Total Internal Reflection Fluorescence)

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The DeltaVision provides the highest quality deconvolution system available on the market. Its advantages are in precision, speed, reproducibility, signal to noise and flexibility.

 

A key extension to the basic DeltaVision system, is the QLM (Quantifiable Laser Module), for FLIP and FRAP based studies. Three lasers are available for use in the system (406, 488 and 535nm).

 

Image Solutions is delighted to be able to offer the QLM capability, that can now be used not only in the classical FLIM/FRAP mode, or to be extended as a TIRF solution.  

 

TIRF microscopy (Total Internal Reflection or evanescent wave microscopy) utilises a laser to selectively excite fluorophores very near a solid surface (within ≤ 100 nm). This is done by projecting a laser beam through the standard optics of the microscope, but angled so that Total internal reflection occurs at the surface of the sample. This is almost exclusively done in an aqueous environment. Using the systems excellent wide field (non confocal mode), the laser beam produces a very thin electromagnetic field, which selectively excites the target fluorophores. The result is an illumination plane that is very thin, highly selective with very low out of focus light (confocal) and high contrast.   

 

The DeltaVison TIRF extension offers not only the flexibility to use the instrument as a low cost TIRF device, but has all of the advantages of the classical DeltaVison functionality in one package!