Onix Flow Control System
The ONIX Perfusion System was developed by CellASIC to overcome the limitations encountered with traditional microfluidics setups. Operations that used to take expert engineers hours of tedious tinkering can now be performed in a few minutes on any benchtop. This system is the equivalent of 8 syringe pumps, a perfusion chamber apparatus, and a CO2 incubator— at a fraction of the cost and greatly improved quality and ease of use.
A low-profile manifold connects the control system with the microfluidic plates. The manifold is set on top of the wells of the plate, and forms an air tight seal using a vacuum mechanism. The wells are addressed with pneumatic pressure to drive liquids into the microfluidic channels. There is no contact between the flow system and the biological solutions (on the microfluidic plate), preventing contamination and lengthy clean-up. Various manifold layouts are available to address different plate formats and application types.
• 8 channel perfusion system |
Yeast Microfluidic Plates
The Yeast Microfluidic plates are optimised for time lapsed imaging of yeast cells with solution exchange. The yeast plates have a unique cell trapping region that holds the yeast cells in a uniform focal plane for time-lapse cell microscopy during perfusion flow. Four upstream solution reservoirs allow rapid switching of the exposure solution during time-lapsed imaging to monitor real time cell responses.
The Y-series plates offer variable trapping heights, as well as special plates for Diploid yeast. Tracking daughter cells is easy due to cells dividing in the same (in focus) plane, which in turn is trapped in between 2 optical glass cover-slips. Another benefit of the trapped cells is that they don't move in and out of focus meaning that they are ideal for long time-lapse movies.
Features and Benefits
- Elastic trap sites holds cells in single foal plane for 16+ hours
- 4 independant chambers under a single imaging window
- Laminar flow switching between 6 inlet solutions
- 170 um thick glass bottom for high quality imaging.
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